Micro and Nano Fluidics Areas of Research:
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Micro and Nano Fluidics:
Biological Interactions
THE EFFECTS OF FLOW AND MASS TRANSFER ON APPARENT REACTION KINETICS
We investigate both experimentally and
theoretically the effects of various flow conditions on the reaction kinetics
between analytes in solution and ligands immobilized to conduits' and
sensors' surfaces and to beads. The ligands are either attached directly
to the solid's surface, embedded in a dextran layer, or embedded in a
lipid bi-layer. The experiments are carried out by our collaborators,
Chaiken's group at UPenn's medical school, in a BIACore biosensor that
utilizes surface plasmon resonance waves to detect the added mass of the
analyte in nearly real time. The mathematical modeling and simulations
are carried out using finite elements codes.
LATERAL FLOW AND SEDIMENTATION REACTORS
Simulation tools have been developed to predict and optimize the performance of bead-based lateral, through flow, and sedimentation immunoassays.
REFERENCES
- Qian, S. and Bau, H., H., 2003, A Mathematical Model of Lateral Flow Bio-Reactions Applied to Sandwich Assays, Analytical Biochemistry 322, 89-98.
- Qian S., and Bau, H., H., 2004, Analysis of Lateral Flow Bio-detectors: Competitive Format, Analytical Biochemistry 326, 211–224.