Lab on Chip (LOC)
The micro/nano fluidics laboratory is engaged in the development of diagnostic devices for disease detection at the point of care. Similar devices can be used for monitoring the quality of the water and food supply.
Point Detection of Pathogens in Oral Samples via UP-Converting Phosphor Transfer
In collaboration with colleagues at the NYU School of Dental Medicine, Leiden University, and Lehigh University, and with support from the NIH-NIDCR, we are developing an integrated system for the detection of pathogens such as HIV in oral fluids at the point of sample collection. The system consists of a disposable sample collector, a disposable cassette, and an analyzer. The system facilitates multiple analysis paths for the processing and identification of antibodies, antigens, DNA, and RNA. The cassette contains flow conduits and reaction chambers for cell lysis, DNA isolation, PCR, and detection. The detection is facilitated through the use of membranes modified with appropriate surface chemistries and Up-converting phosphors that absorb infrared energy and subsequently emit it as visible light.
A Cassette for the Processing and Detection of Nucleic Acids and Proteins
Links
http://www.nyu.edu/projects/mod/index.html
Publications
Qian, S. and Bau, H., H., 2003, A Mathematical Model of Lateral Flow Bio-Reactions Applied to Sandwich Assays, Analytical Biochemistry, 322, 89-98.
Qian S., and Bau, H., H., 2004, Analysis of Lateral Flow Bio-detectors: Competitive Format, Analytical Biochemistry 326, 211–224.
Chen, Z., Qian, S., Abrams, W., R., Malamud, D., and Bau, H., H., 2004, Thermosyphon-based PCR Reactor: Experiment and Modeling, Analytical Chemistry, 76, 3707-3715
Qian, S. Burger, R., and Bau, H., H., 2005, Analysis of Sedimentation Biodetectors, Chemical Engineering Science, 60, 2585 – 2598.
Qian, S. Burger, R., and Bau, H., H., 2005, Analysis of Sedimentation Biodetectors, Chemical Engineering Science, 60, 2585 – 2598.
Malamud, D., Bau, H., H., Niedbala, S., Corstjens, P., 2005, Point Detection of Pathogens in Oral Samples, Adv Dent Res 18:12-16.
Wang, J., Chen, Z., Qian S., and Bau, H. H., 2005, Thermally-Actuated, Phase-Change Flow Control for Microfluidic Systems, Lab on Chip, 5, 1277 – 1285
Wang, J., Chen, Z., Mauk, M., Hong, K-S, Li, M., Yang, S., and Bau, H., H., 2005, Self-Actuated, Thermo-Responsive Hydrogel Valves for Lab on a Chip, Biomedical Microdevices 7 (4), 313-322.
Z. Chen, J. Wang, M. G. Mauk, H. H. Bau, C. Davis, G. Tong, F. Winslow, W. R. Abrams, D. Malamud, 2005, On Chip PCR Amplification with Integrated Lateral Flow Detection, no. 0240, µTAS 2005, Boston, Massachusetts, USA, October 913th, 2005, 1297-1299
Z. Chen, P.L.A.M. Corstjens, M. Zuiderwijk, J. Wang, M. G. Mauk, H. H. Bau, W. R. Abrams, D. Malamud, 2005, Phosphor Reporter Particle-Based Disposable Device for the Rapid Detection of HIV at the Point of Care, no. 0241, µTAS 2005, Boston, Massachusetts, USA, October 913th, 2005, 791-793.
Wang, J., Mauk, M.G., Chen, Z., Bau, H.H., 2005, “A disposable Microfluidic Device for Cell Lysis and DNA Isolation”, no. 0345, Proceedings of ?TAS 2005, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Boston, Massachusetts, USA, K.F. Jensen, J. Han, D. J. Harrison, and J. Voldman (editors) October 9th 13th, 2005, pp 289-291.
Wang, J., Chen, Z., Corstjens, P. L. A. M., Mauk, M., and Bau, H. H., 2006, A Disposable Microfluidic Cassette for DNA Amplification and Detection, Lab on Chip, 6, 46-53
Ziober, B., Mauk, M., Chen, Z. and Bau, H., H., 2007, Lab-on-a-Chip for Oral Cancer Screening and Diagnosis, accepted for publication in Head & Neck (HED-06-0558.R1)
Abrams, W. R., C. A. Barber, K. McCann, G. Tong, Z. Y. Chen, M. G. Mauk, J. Wang, A. Volkov, P. Bourdelle, P. L. A. M. Corstjens, M. Zuiderwijk, K. Kardos, S. Li, H. J. Tanke, R. S. Niedbala, D. Malamud and H. H. Bau (2007). "Development of a Microfluidic Device for Detection of Pathogens in Oral Samples Using Upconverting Phosphor Technology (UPT)." Annals of the New York Academy of Science 1098: 375-388.
Chen, Z., M. G. Mauk, J. Wang, W. R. Abrams, P. L. A. M. Corstjens, R. S. Niedbala, D. Malamud and Haim H.Bau (2007). "A Microfluidic System for Saliva-Based Detection of Infectious Diseases." Annals of the New York Academy of Science 1098: 429-436.
Corstjens, P. L. A. M., Z. Chen, M. Zuiderwijk, H. H. Bau, W. R. Abrams, Daniel Malamud, R. S. Niedbala and H. J. Tanke (2007). "Rapid Assay Format for Multiplex Detection of Humoral Immune Responses to Infectious Disease Pathogens (HIV, HCV, and TB)." Annals of the New York Academy of Science 1098: 437-445.
Mauk, M. G., B. L. Ziober, Z. Chen, J. A. Thompson and Haim H. Bau (2007). "Lab-on-a-Chip Technologies for Oral-Based Cancer Screening and Diagnostics: Capabilities, Issues, and Prospects." Annals of the New York Academy of Science 1098: 467.